In der vorliegenden Arbeit wird eine verbesserte Biokristallisationsmethode präsentiert. Die Methode kann für die Untersuchung der pictomorphologischen Eigenschaften, d.h. der morphologischen Charakteristika von Kristalltexturen, von biologischen Proben angewendet werden. Die Methode wird in der medizinischen Forschung und in Ergänzung zu chemischen Analysen in der landwirtschaftlichen Forschung zur Qualitätsprüfung angewendet. Das Ziel der vorliegenden Untersuchung lag in der Verbesserung existierender Kristallisationskammern durch Kontrolle physikalischer Faktoren, welche die Evaporation und Kristallisation beeinflussen können. Die Reproduzierbarkeit der Methode wurde zweifach untersucht: 1) Die Kontrolle physikalischer Faktoren wurde durch Variationen der Temperatur und Luftfeuchtigkeit in drei vergleichbaren Versuchen untersucht, die über 17 Stunden verliefen. 2) Die Reproduzierbarkeit der Kristalltexturen wurde durch Klassifikationen von 32 kodierten Kristallogrammen anhand von Referenzkristallogrammen in sieben Klassen untersucht. Die Klassifikation wurde von drei Personen vorgenommen. Die Kristallogramme wurden unter Zuhilfenahme unterschiedlicher Polymerisierungsraten und Konzentrationen von wäßrigen PVP-Lösungen (Polyvinylpyrrolidon) hergestellt. Im Vergleich zu einem früheren Experiment konnte die Kontrolle der Temperatur und Luftfeuchtigkeit wesentlich verbessert werden. Die Ergebnisse zeigten eine relativ hohe positive Bestimmungsrate der Klassifikationen (69-75%) für die drei Personen. Die Korrelationskoeffizienten dieser Klassifikationen waren hoch (r = 091—093). Die Resultate wiesen ferner auf eine starke Beziehung der PVP-Polymerisierungsrate und der morphologischen Charakteristika der Kristalltextur hin.
An experimentally refined biocrystallization method is presented. The method is applicable for investigating the pictomorphological properties of biological samples, as expressed in morphological features of crystal textures. The method is applied in medical research, and in agricultural research concerning crop quality, as a complement to chemical analyses of single compounds. The objective of the present study was to refine existing crystallization chamber techniques through control of physical factors influencing evaporation and crystallization. The reproducibility of the method was studied in a two-fold manner: l) Concerning the control of physical factors variations in air temperature and humidity were studied during three similar experiments With experimental periods of 17 hours. 2) Concerning the reproducibility of textural features three investigators performed a visual classification of 32 coded crystallograms produced during the experiments, relative to a reference set of seven classes. The crystallograms were produced on the basis of aqueous solutions of the synthetic polymer PVP (Polyvinylpyrrolidone), from different polymerization and concentration levels. Results indicated markedly improved control of air temperature and humidity conditions, relative to a previous study. Results indicated relatively high correct classification scores for all three investigators (69—75%). The correlation coefficients for the 32 0bservations were high (r = 091—093). Furthermore results indicated a strong correlation between polymerization level of PVP and morphological features.
An experimentally refined biocrystallization method is presented. The method is applicable for investigating the pictomorphological properties of biological samples, as expressed in morphological features of crystal textures. The method is applied in medical research, and in agricultural research concerning crop quality, as a complement to chemical analyses of single compounds. The objective of the present study was to refine existing crystallization chamber techniques through control of physical factors influencing evaporation and crystallization. The reproducibility of the method was studied in a two-fold manner: l) Concerning the control of physical factors variations in air temperature and humidity were studied during three similar experiments With experimental periods of 17 hours. 2) Concerning the reproducibility of textural features three investigators performed a visual classification of 32 coded crystallograms produced during the experiments, relative to a reference set of seven classes. The crystallograms were produced on the basis of aqueous solutions of the synthetic polymer PVP (Polyvinylpyrrolidone), from different polymerization and concentration levels. Results indicated markedly improved control of air temperature and humidity conditions, relative to a previous study. Results indicated relatively high correct classification scores for all three investigators (69—75%). The correlation coefficients for the 32 0bservations were high (r = 091—093). Furthermore results indicated a strong correlation between polymerization level of PVP and morphological features.