Laboratory intercomparison for biocrystallization (crystallization with additives) applied to different wheat varieties

Elemente der Naturwissenschaft 102, 2015, P. 5-13 | DOI: 10.18756/edn.102.5


After the biocrystallization method has been documented, standardized and applied on various agricultural and food samples, laboratory intercomparison is performed on wheat samples. Standardized and non‑standardized approaches in five different laboratories throughout Europe are compared based on their ability to group coded samples according different wheat varieties as classes. The grouping is based on defined criteria from visual evaluation of the crystallization patterns. All laboratories can successfully group coded samples according to defined classes. This indicates a robust method principle, which needs further research work. Next research challenges may be investigations on the influence of different factors along the laboratory process as well as insights in the methods principle.

  • Busscher, N., Kahl, J., Ploeger, A. (2014): From needles to pattern in food quality determination. Journal of the Science of Food and Agriculture, 94, S. 2578‑2581.
  • Busscher, N., Kahl, J., Andersen, J.‑O., Huber, M., Mergardt, G., Doesburg, P., Paulsen, M., Ploeger, A. (2010a): Standardization of the biocrystallization method for carrot samples. Biological Agriculture & Horti- culture, 27 (1), S. 1‑23.
  • Busscher, N., Kahl, J., Doesburg, P., Mergardt, G., Ploeger, A. (2010b): Evaporation influences on the crystallization of an aqueous dihydrate cupric chloride solution with additives. Journal of Colloid and Interface Sciences, 334 (2), S. 556‑562.
  • Doesburg, P., Huber, M., Andersen, J.-O., Athmann, M., van der Bie, G., Fritz, J., Geier, U., Hoekman, J., Kahl, J., Mergardt, G., Busscher, N. (2014): Standardization and performance of a visual Gestalt evaluation of biocrystallization patterns reflecting ripening and decomposition processes in food samples. Biological Agriculture & Horticulture, published online 24.12.2014, DOI: 10.1080/01448765.2014.993705
  • Doesburg, P., Nierop, A.F.M. (2013): Development of a structural image analysis approach on strutures from CuCl2·2H2O crystallization with agricultural products. Computer and Electronics in Agriculture, 90, S. 63‑67.
  • Fritz, J., Athmann, M., Kautz, T., Köpke, U. (2011): Grouping and classi- fication of wheat from organic and conventional production systems by combining three image forming methods. Biological Agriculture & Horticulture, 27, S. 320‑336.
  • Huber, M., Andersen, J.-O., Kahl, J., Busscher, N., Doesburg, P., Mergardt, G., Kretschmer, S., Zlecka, A., Meelursarn, A., Ploeger, A., Nierop, D., van de Vijver, L., Baars, E. (2010): Validation of the visual evaluation of biocrystallizations. Development of a reliable and valid instrument for visual evaluation according to ISO‑Norms for sensory analyses. Biological Agriculture & Horculture, 27 (1), S. 25‑40.
  • Kahl, J., Busscher, N., Hoffmann, W., Mergardt, G., Clawin-Raedecker, I., Ploeger, A. (2014a): Differentiation of milk fractions and polyvinylpyrrolidone with different molecular weight by patterns derived from cupric chloride crystallization with additives. Analytical Methods, 6, S. 3173‑3176.
  • Kahl, J., Busscher, N., Mergardt, G., Mäder, P., Torp, T., Ploeger, A. (2015): Crystallization with additives applied to winter wheat cultivars from a controlled field trial. Journal of the Science of Food and Agriculture, 95, S. 53‑58.
  • Kahl, J., Busscher, N., Mergardt, G., Andersen, J.-O., Doesburg, P., Meelursarn, A., Ploeger, A. (2014b): Standardization and performance test of crystallization with additives on wheat samples. Food Analytical Methods, in press, DOI 10.1007/s12161‑015‑0142‑6.
  • Kahl, J., Busscher, N., Hoffmann, W., Mergardt, G., Clawin-Raedecker, I., Kiesner, C., Ploeger, A. (2013a): Development and performance of crystallization with additives applied on different milk samples. Food Analytical Methods, 7, S. 1373‑1380.
  • Kahl, J., Bodroza-Solarov, M., Busscher, N., Hajsolva, J., Kneifel, W., Kokornaczyk, M., van Ruth, S., Schulzova, V., Stolz, P. (2013b): Status quo and future research challenges on organic food quality determination with focus on laboratory methods. Journal of the Science of Food and Agriculture, 94, S. 2595‑2599.
  • J. Kahl, T. Baars, S. Bügel, N. Busscher, M. Huber, D. Kusche, E. Rembialkowska, O. Schmid, K. Seidel, B. Taupier-Letage, A. Velimirov, A. Zalecka (2012): Organic food quality: a framework for concept, definition and evaluation from the European perspective. Journal of the Science of Food and Agriculture, 92, S. 2760‑2765.J. Kahl, N. Busscher, A. Ploeger (2010): Questions to the validation of holistic methods testing organic food quality. Biological Agriculture & Horticulture, 27 (1), S. 81‑94.
  • J. Kahl, N. Busscher, P. Doesburg, G. Mergardt, M.Huber, A. Ploeger (2009): First tests of standardized biocrystallization on milk and milk products. European Food Research and Technology, 229, S. 175‑178.
  • K. Seidel, J. Kahl, F. Paoletti, I. Birlouez, N. Busscher, U. Kretzschmar, M. Särkkä-Tirkkonen, R. Seljasen, F. Sinesio, T. Trop, I. Baiamonte (2013): Quality assessment of baby food made of different pre-processed organic raw materials under industrial processing conditions. Journal Food Science and Technology, 52(2), S. 803‑812.
  • M. Szulc, J. Kahl, N. Busscher, G. Mergardt, P. Doesburg, A. Ploeger (2010): Discrimination between organically and conventionally grown winter wheat farm pair samples using the copper chloride crystallisation method in combination with computerised image analysis. Computer and Electronics in Agriculture, 74, S. 218-222.