Cupric Chloride Crystallisation in the Presence of Additive: Experimental Conditions and Developmental Perspectives

Elemente der Naturwissenschaft 111, 2019, P. 46-55 | DOI: 10.18756/edn.111.46


The experimental conditions of the copperchloride crystallisation method in presence of additives was recently rewiewed. A new operating procedure allowed improving the homogeneity of a crystallogram set as well as the discriminating power of the method. Notwithstanding reducing the spread of the appearance times of the first seed (t1), the phenomenon is still polarised. Correctiv options are proposed as well as technical simplifications, which may improve the practicability of the method. Miniaturization of the method would also help to simplify its implementation and could facilitate the exploitation of results.

  • Andersen, J.O., Laursen, J., Kolster, P. (1998): A refined biocrystallization method applied in a pictomorphological investigation of a polymer. Elemente d. N. 68, pp. 1–20.
  • Barth, J.G., Roussaux, J., Suppan, K., Rosa dos Santos, S. (2011): Crystallisation of a film of copper chloride in the presence of additives. Preliminary study on the experimental conditions and criteria of quality. Elemente d. N. 94, pp. 69–99.
  • Barth, J.G., Roussaux, J., Wilkens, A., Jacobi, M. (2013): Techniques for washing the supports used for copper chloride crystallisation with additive. Elemente d. N. 98, pp. 5–19.
  • Baumgartner, S., Doesburg, P., Scherr, C., Andersen, J.O. (2012): Development of a biocrystallisation assay for examining effects of homeopathic preparations using cress seedlings. Evidence-Based Complementary and Alternative Medicine Vol. 2012, Art. ID 125945, pp. 1–14, DOI:10.1155/2012/125945.
  • Doesburg, P., Nierop, A.F.M. (2013): Development of a structure analysis algorithm on structures from CuCl2, 2H2O crystallization with agricultural products. Computers and Electronics in Agriculture 90, pp. 63–67.
  • Fritz, J., Athmann, M., Kautz, T., Köpke, U. (2011): Grouping and classification of wheat from organic and conventional production systems by combining three image forming methods. Biological Agriculture & Horticulture, 27, pp. 320–336.
  • Kahl, J. (2007): Entwicklung, in-house Validierung und Anwendung des ganzheitlichen Verfahrens Biokristallisation für die Unterscheidung von Weizen-, Möhren- und Apfelproben aus unterschiedlichem Anbau und Verarbeitungsschritten. Habilitationsschrift, Université de Kassel;
  • Piva, M.T., Lumbroso, S., Sieso, V., Monnin, E., Mion, H., Blanc, F., Magnan de Bornier, B. (1994): Cupric chloride crystallization with human blood. Study of pictures obtained in different pathologies. Elemente d. N. 61, pp. 25–39.
  • Reiter, G., Barth, J.G. (2010): Some general remarks on crystallisation in the presence of additives. Elemente d. N. 92, pp. 39–61.