Articles

Cupric chloride crystallization with human blood.

Study of pictures obtained in different pathologies
Marie-Thérèse Piva, Serge Lumbroso, Victor Sieso, Eric Monnin, Henri Mion, Francois Blanc, Bernard Magnan de Bornier
Elemente der Naturwissenschaft 61, 1994, S. 25-39 | DOI: 10.18756/edn.61.25
Colloquium | Sprache: English | €6.00
Artikelreferenz exportieren

Zusammenfassung:

We have studied the different pictures that appear after cupric chloride crystallization in the presence of blood in a bidimensional circular plane system. We describe the method in its various technical aspects and define the optimal conditions to obtain these pictures. In healthy subjects (chosen as controls), the crystallization was more regular than in non-healthy subjects. In 80 patients classified into 8 pathological groups there were crystallization alterations leading to geometrical figures. The different figures obtained are listed and schematized. An experimental procedure with less complex media than blood (i.e amino-acid solutions, serum albumin dilutions) demonstrates the influence of protein in eliciting forms. The statistical study showed significant differences between most of the patient groups and a relation between a single alteration »Form« frequency and an etiological group. Some crystallization patterns were also correlated with known variations of some different seric proteins. This report shows a one to one relationship between pathology and pattern allowing one to »visualize« a pathological state on a crystallographic picture.

Referenzen
  • Barth, J.-G. (1990): Empfindliche Kristallisation - Krebs und Präkanzerose. Elemente d. N. 52, p. 42 - 51.
  • Chebotareva, V.D., Maidannicle, V.G., Paderno, V.N., Polerasen, N.M. and Martynenleo, A.N. (1990): Diagnostic potentialities of the crystallooptic method in children with pyelonephritis. Pediatria, 5, p. 46—51 (in Russian).
  • Cocude, M., Barth, ].-G, Brayet, B. et Francois, P. (1992): La pneumoconiose des houilleurs et son suivi médical. La méthode des cristallisations sensibles au banc d’essai. Mines et Carriéres. Industrie minérale, 74, p. 41—47.
  • Frot, J. C., Hofmann, H., Muller, F., Benazet, M.-F. et Giraudet, P. (1984): Le concept du profil protéique. Ann. Biol. Clin., 42, p. 1-8.
  • Giraudet, P., Coudod, B. und Postel, P. (1990): Exploitation clinique des profils protéiques. Technicon.
  • Goldbeter, A. (1990): Rythmes et chaos dans les systémes biologiques et cellulaires. Ed. Masson, Collection Biologie Théorique.
  • Hoffmann, C. (1985): Test de cristallisation sensible avec du sang en rapport avec le cancer. These médecine 303, Université Louis Pasteur, Strasbourg.
  • Layle, M. F. et Engler, R. (1984): Les différents profils des variations des protéines plasmatiques dans les états inflammatoires. Path. Biol., 22, p. 645-650.
  • Laurent, P., Figaeroa, N. et Zeller, H. (1980): Néphélométrie, principes, applications a l’immunoprécipitation en milieu liquide. Rev. inst. Pasteur Lyon, 13(2), p.153-179.
  • Leray, J. (1968): Growth Kinetics of hydrated cupric chloride. J. Crystal Growth, 3,4, p. 344—349.
  • Leray, J. (1970): Etude géométrique des configurations engendrées par la cristallisation dendritique de solutions salines concentrées. Bull. Soc. Fr. Mineral. Cristallogr., 93, p. 201-212.
  • Leray, J. (1973): Profil de la surface libre d’un film liquide hétérogéne. J. de Chimie Physique, 10, p. 1428—1485.
  • Macart, M. and Gerbaud, L. (1982): An improvement of the Coomassie blue dye binding method allowing an equal sensitivity to various proteins: application to cerebrospinal fluid. Clin. Chim. Acta, 122, p. 93—103.
  • Meliconi, F., Parracino, 0. and Facchini, A. (1988): Acute phase proteins in chronic and malignant liver diseases. Liver, 8(2), p. 65-74.
  • Neuhaus, A. (1957): Die kristallographischen Grundlagen der »Diagnostischen Kristallisationen«. p. XIV-XL. In Selawry, A. und Selawry, O.: Die Kupferchlorid—Kristallisation in Naturwissenschaft und Medizin. Stuttgart.
  • Nickel, E. (1968): Die Reproduzierbarkeit der sogenannten»empfindlichen Kupferchloridkristallisation«. Bull. Soc. Frib. Sc. Nat., 2(II), p. 65—179.
  • Pfeiffer, E. (1931): Studium von Formkräften an Kristallisationen. Dresden.
  • Pfeiffer, E. (1975): Sensitive Crystallisation Processes, second Printing, New York.
  • Selawry, A. und Selawry, O. (1957): Die Kupferchlorid—Kristallisation in Naturwissenschaft und Medizin. Stuttgart.
  • Shibata, T., Takakuwa, Y., Obata, H., Tanaka, A., Kobure, M. (1993): A blood crystallisation method for diagnosis, test of treatment effect, severity, and early discrimination of diseases for the aged. Annual Report of Comprehensive Research Project on Aging and Health Vol.6. Central Office for the Research Grant for Comprehensive Research Project on Aging and Health, Tokyo, in press (in ]apanese).
  • Thom, R. (1977): Stabilité structurelle et morphogenése. Paris.
  • Turing, A. M. (1952): The chemical basis of morphogenesis. Phil. Trans. R. Soc. Lond., B237, 37.
  • Van Kampen, E. J. and Ziljstm, W. G. (1961): Standardization of hemoglobinometry H. The hemoglobincyanide method. Clin. Chim. Acta, 6, p.538—544.
  • Vidal, C. (1989): Les réactions oscillantes en chimie. La Recherche, 20, 216. p.1478—1485.